Research unit of Gamete preservation

Project leader

 Assoc.Prof.Dr. Kaywalee Chatdarong (DVM, MS.c., Ph.D.)

                The research study related to gamete preservation will mainly focus on the cryobiology of gamete cells and reproductive tissue i.e. sperm, oocytes, testicular and ovarian tissues in companion animals, live and post-mortem endangered wildlife species. The purpose is to improve scientific understanding in this field and to apply this knowledge to the benefit of reproductive management and establishment of genome resource bank. Together with cryobiology research, the other assisted reproductive techniques i.e. in vitro embryo production, culture and laparoscopic embryo transfer will be integrated with the ultimate goal to produce the new offspring especially in endangered wildlife conservation research area.

Mission

To understand and investigate effect of low temperature to gamete cell and reproductive tissue function and survival

To develop high potential short and long term gamete cell and gonadal tissue preservation procedures which are practical under the field condition and in the remote area

To integrate cryobiology research with other assisted reproductive techniques aims to produce the new offspring both in companion and wildlife species

To establish genome resource bank emphasized on  live and post-mortem endangered wildlife species under the collaboration with Thai government agencies such as The Zoological Park Organization and The Royal Forest Department, The Department of National Parks, Wildlife and Plant Conservation, Ministry of Natural Resources and Environment  

Research projects

Effects of cold storage prior to freezing on glutathione peroxidase and superoxide dismutase activities, reactive oxygen species and sperm quality in dogs

Chilling of dog semen in lyophilized tris egg-yolk extender

Cryopreservation of testicular tissue in wild animals post-mortem

Extracellular ATP uptake and its effect on epididymal cat sperm freezing

In vitro growth of pre-antral follicle in domestic and wild cats

Management of gamete rescue in wild animals post mortem

Comparison of cat ovarian tissue freezing using a passive cooling container and vitrification

Staff

Prof.Dr. Kaywalee Chatdarong (DVM, MS.c., Ph.D.)

Paweena Thuwanut (DVM, MS.c., Ph.D.)

Wanlaya Tipkanta (Graduate student; Ph.D. program)

Nae Tanpradit (Graduate student; Ph.D. program)

Contact

                Assoc.Prof.Dr.Kaywalee Chatdarong

Department of Obstetrics, Gynaecology and Reproduction

Faculty of Veterinary Science, Chulalongkorn University

Pathumwan, Bangkok 10330, Thailand.

Tel. +662-218-9644

Fax +662-252-0738

E-mail: kaywalee.c@chula.ac.th